Figure 1: DAXX interacts with PTEN. | Nature Communications

Figure 1: DAXX interacts with PTEN.

From: PTEN regulates glioblastoma oncogenesis through chromatin-associated complexes of DAXX and histone H3.3

Figure 1

(a) Predicted protein–protein interactions between PTEN, TP53, PML, DAXX and MCRS1 using the human protein interaction map. (b) In vitro pull-down using mixed Flag-DAXX and His-PTEN recombinant proteins immunoprecipitated (IP) with anti-PTEN and immunoblotted (IB) with anti-Flag. (c) Co-immunoprecipitation assay using whole cell protein lysates from different cell lines expressing endogenous DAXX and PTEN. IP anti-DAXX and IB anti-PTEN. (d,e) PTEN and DAXX interacting domains were mapped using 293 T cells co-transfected with Flag-Daxx-wt or GFP-PTEN and deletion constructs of each protein.

Back to article page