Figure 5: Drinking water evokes an increased calcium response in PAM-β′1 neurons in thirsty flies.
From: Neural circuits for long-term water-reward memory processing in thirsty Drosophila
(a) GCaMP6 response to water intake in distinct groups of PAM neurons driven by DDC-GAL4 in thirsty (left panel) or water-sated (right panel) flies, respectively. Scale bar, 20 μm. (b,c) Time-lapse recordings of GCaMP6 responses (b) in thirsty (left panel) or water-sated (right panel) flies; the average responses (c) to water intake in distinct groups of PAM neurons driven by DDC-GAL4. Each value represents mean±s.e.m. (N=8 for thirsty flies; N=11 for water-sated flies). NS, not significant (P>0.05). *P<0.05; t-test. (d) GCaMP6 response to water intake in PAM-β′1 neurons driven by VT8167-GAL4 in thirsty (left panel) or water-sated (right panel) flies, respectively. Scale bar, 20 μm. (e,f) Time-lapse recordings of GCaMP6 responses (e) in thirsty (left panel) or water-sated (right panel) flies; the average responses (f) to water intake in PAM-β′1 neurons driven by VT8167-GAL4. Each value represents mean±s.e.m. (N=8 for thirsty flies; N=10 for water-sated flies). *P<0.05; t-test.
