Figure 5: FRS7 and FRS12 integrate a transcriptional repressor complex.
From: The transcriptional repressor complex FRS7-FRS12 regulates flowering time and growth in Arabidopsis
(a) Spoke-model of the protein interaction network identified for FRS12 by TAP–MS. The dashed edges represent previously unknown interactions. Blue nodes represent preys and the grey node represents the bait protein. (b) BiFC analysis in N. benthamiana leaves of FRS7 and FRS12 nuclear interactions. Scale bars, 50 μm. (c) Transient trans-activation assay in tobacco protoplasts co-transfected with a ProUAS:fLUC reporter construct, effector constructs (Pro35S:DBD-FRS12 and Pro35S:DBD-FRS7) and a Pro35S:rLUC construct to normalize expression. Mean expression values±s.e.m. are depicted relative to the respective Pro35S:GUS-DBD control effector construct; n=8, *P<0.05, t test. (d) GO-enrichment network of the genes identified by RNA-Seq as repressed (fold change ≥2) in the Pro35S:FRS12-GR-1 line. (e) Heat map representing the genes identified by RNA-Seq as differentially regulated (fold change ≥2) in the Pro35S:FRS12-GR-1 line. Indicated genes are associated to stress responses, flowering time, light signalling, growth or circadian rhythms.
