Figure 3: Microarray analysis of breast lesions and TAM-treated ER-Src cells.

(a) Heatmaps of ABP deregulated in (left panel) ER-Src cells treated with TAM for the different time points indicated¹⁸ or (right panel) ADH and/or DCIS and their expression in the three IDC grades (IDC1, IDC2, IDC3). Fold-changes are in Log2R. (Middle panel) Common ABPs misregulated in the same direction in ADH/DCIS and in TAM-treated ER-Src cells. Orange and blue indicate genes up- and downregulated, respectively. (b) (Left) Schematic of the multistep model of breast cancer progression. (Right) Pathway impact analysis for the sets of genes differentially expressed in ER⁺ ADH, ER⁺ DCIS, ER⁺ IDC1, ER⁺ IDC2, ER⁺ IDC3, ER⁻ IDC2 and ER⁻ IDC3 when compared to normal breast tissue samples. All pathways significantly affected were plotted on the basis of their impact factor. Black arrows indicate the ‘Regulation of actin cytoskeleton’ pathway (magenta). See also Supplementary Table 1, Supplementary Data 1 and 2 and Supplementary Fig. 4.