Figure 1: HLA-DP molecules whose β-chains encode Gly⁸⁴ are unable to present CLIP.

Surface class II, Ii and CLIP expression on T2 transfectants were analysed by flow cytometry following staining with specific monoclonal antibodies (mAbs). Note that T2 cells constitutively express Ii. (a,b) T2 cells were stably transduced with DPA1*01:03 (DPA1; a) or DPA1*02:01 (DPA2; b) in conjunction with DPB1*02:01(DPB2), DPB1*04:01 (DPB4), DPB1*05:01 (DPB5) or DPB1*08:01 (DPB8). (c) T2 cells were stably transfected with DPA1 along with mutated DPB2^84DEAV87 (DP2^84DEAV87), DPB4^84DEAV87 (DP4^84DEAV87), DPB5^84GGPM87 (DP5^84GGPM87) or DPB8^84GGPM87 (DP8^84GGPM87), whose amino-acid residues at positions 84–87 of the DPβ chain were substituted as indicated. (d) T2 cells were stably transfected with DPA1 in conjunction with mutant DPB2^84Asp (DP2^84Asp), DPB4^84Asp (DP4^84Asp), DPB5^84Gly (DP5^84Gly) or DPB8^84Gly (DP8^84Gly), where point mutations of the amino-acid residue at position 84 of each DPβ chain were substituted as indicated.