Figure 2: Identification of the LBS in Mic60.
From: Regulated membrane remodeling by Mic60 controls formation of mitochondrial crista junctions
(a,b,e–i) Liposome sedimentation assays (left) and electron micrographs (right) of Folch liposomes incubated with the indicated Mic60 constructs. Arrows indicate tubule branches. Scale bars, 2 μm and 500 nm within the close-ups, respectively. (c) Sequence alignment of the predicted LBS in Mic60, with secondary structure predictions on top. Sequences of Mic60 from C. thermophilum (ct), S. cerevisiae (sc), H. sapiens (hs), M. musculus (mm), B. Taurus (bt), G. gallus (gg), F. catus (fc) and A. thaliana (at) were aligned. Positively charged residues in blue; negatively charged residues in red, hydrophobic residues in green and uncharged residues in yellow. (d) Helical wheel projections of the LBS1 from C. thermophilum (ct_LBS1) and S. cerevisiae (sc_LBS1) indicate the amphipathic nature of the predicted α-helix. The red asterisks mark residues mutated in this study.
