Figure 1: MCAM/FGF4-dependent apical surface biogenesis. | Nature Communications

Figure 1: MCAM/FGF4-dependent apical surface biogenesis.

From: The signalling receptor MCAM coordinates apical-basal polarity and planar cell polarity during morphogenesis

Figure 1

(a) Upper panel, cartoon of MCAM-BD 1–3. Lower panel, yeast zygotes obtained after mating the bait strain containing pGBKT7-MCAM with the library strain containing pGADT7-FGF2, FGF4, and FGF8. BD, DNA-binding domain; AD, activation domain; +, positive control with p53-BD; and −, negative control with empty AD vector and BD-lambda. (b) Co-immunoprecipitation of MCAM/FGF4 and FGFR1/FGF4 with the protein lysate treated with or without the heparinase I and heparinase III (0.06 IU ml−l). (c) Kinetic dissociation constant (KD) of FGF4/MCAM, FGF4/FGFR1, or FGF4/ FGFR2 complexes was measured using a surface plasmon resonance method. (d) Distribution of polarized MCAM and unpolarized FGFR1 on chemotaxing cells. The source concentration of FGF4 in the chemotaxis assay is 10 ng ml−l. (e) Co-localization of endogenous MCAM and the apical marker aPKCζ in chemotaxing cells. Scale bar, 20 μm. (f,g) Time-lapse live-cells imaging of endogenous MCAM (f) or exogenous MCAM-RFP (g) at the leading edge of chemotaxing cells.

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