Figure 5: MCAM controls activation of NFAT. | Nature Communications

Figure 5: MCAM controls activation of NFAT.

From: The signalling receptor MCAM coordinates apical-basal polarity and planar cell polarity during morphogenesis

Figure 5

(a) NFAT-Luc reporter assay in HEK293 cells transfected together with MCAM RNAi or MCAM expression vector. RLU means relative luciferase activity. Data are presented as mean±s.e.m. (n=3). One-way ANOVA with Tukey’s post-test and **P value<0.01. (b) After co-transfection of reporter plasmids and RNAi for 20 h, cells were cultured with serum-free medium overnight. NFAT-Luc activity was measured following 1 h treatment with FGF4 (2 ng/ml). No treatment served as negative control. Data are presented as mean±s.e.m. (n=3). Two-way ANOVA with Bonferroni post-test and ***P value<0.001. (c) Immunoprecipitation of MCAM followed by immunoblotting (IB). (d) PLC-γ abundance in membrane fraction of HEK293 cells and in whole cell lysate after a 48 h transfection with RNAi with or without treatment with FGF4 for 1 h. (e) Model of MCAM-controlled NFAT activation responding to FGF4. Once dimerization, MCAM conjugates FYN kinase to activate PLC-γ.

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