Table 1 Kinetic measurements for experiments illustrated in Fig. 4.

From: The cohesin-like RecN protein stimulates RecA-mediated recombinational repair of DNA double-strand breaks

Order of addition

Average ATP hydrolysis rate (μM min −1 )±s.d.

Average lag time (min)±s.d.

1—RecN, no DNA, no RecA K83R

8.3±1.7

0

2—RecN+DNA, no RecA K83R

13.7±4.1

0

3—RecN+DNA→RecA K83R

53.4±4.2

25.0±5.0

4—RecN+RecA K83R→DNA

47.5±8.5

34.3±5.3

5—RecA K83R+DNA→RecN

56.3±15.6

8.3±6.7

RecA K83R+DNA, no RecN

0.20±0.17

0

  1. RecN ATP hydrolysis rate (in μM min−1) and lag times (in min) for the experiments illustrated in Fig. 4. The order of additions match the numbered reactions. The ATP hydrolysis rates were measured after the reaction reached the steady state. Lag times, where applicable, represent the time required for the reaction to reach a steady-state rate of hydrolysis after the addition of all reaction components (Fig. 4). All averages and s.d.’s were calculated from 4 independent trials except that data for reaction 5 were calculated from 10 independent trials.
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