Table 2 Steady-state RecN ATP hydrolysis rates under D-loop assay conditions.

From: The cohesin-like RecN protein stimulates RecA-mediated recombinational repair of DNA double-strand breaks

Order of addition

n

Probe DNA (μM nt)

Target DNA (μM nt)

Average ATP hydrolysis rate (μM min −1 )±s.d.

RecN+target DNA control (reaction 1, Fig. 5b)

3

0

10

2.4±0.5

RecN+probe DNA control (reaction 2, Fig. 5b)

3

10

0

2.4±0.4

RecN+target and probe DNA control (reaction 3, Fig. 5b)

3

10

10

2.3±0.5

RecA K83R+probe DNA→RecN (reaction 4, Figs 5b and 6a)

9

10

0

4.5±1.8

RecA K83R→RecN+target DNA (reaction 5, Figs 5b and 6a)

9

0

10

30.9±3.2

RecA K83R+probe DNA→RecN+target DNA (Fig. 6a)

5

1

10

27.3±1.7

 

8

2.5

10

28.5±1.7

 

5

5

10

36.1±4.2

 

3

7.5

10

39.7±2.4

 

5

10

1

7.7±4.7

 

8

10

2.5

14.5±7.4

 

3

10

5

29.2±9.3

 

5

10

7.5

38.6±2.5

RecA K83R+probe DNA→RecN+target DNA (reaction 6, Figs 5b and 6a)

15

10

10

49.2±6.4

RecA K83R+probe DNA→RecN+heterologous target DNA (reaction 7, Fig. 5b)

3

10

10

48.3±1.4

RecA K83R+probe DNA→RecN+linearized, homologous target DNA (Fig. 6b, 2.4 kb target DNA)

6

10

10

47.9±5.0

  1. Reaction conditions are described in the legend to Fig. 5. Averages and s.d.’s were calculated from the number of independent trials indicated (n). Order of addition describes the experimental condition and the relevant figure containing representative data. The probe and target DNAs are derived from 2.4 kbp plasmid DNA and are described in the legend to Fig. 2. Heterologous DNA is non-homologous, supercoiled RF1 φX174 DNA.
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