Figure 1: hCINAP promotes invasiveness and metastasis in CRC cells. | Nature Communications

Figure 1: hCINAP promotes invasiveness and metastasis in CRC cells.

From: Adenylate kinase hCINAP determines self-renewal of colorectal cancer stem cells by facilitating LDHA phosphorylation

Figure 1

(a) Western blot analysis of the hCINAP protein levels in human CRC and adjacent paired normal tissues, normalized to Actin (n=50). See also Supplementary Fig. 1. (b) The wound-healing assay using SW480 cells expressing the indicated shRNAs and plasmids. Scale bar, 100 μm. (c) Quantification of the migration potential and proliferation rate of the cells in the migration assay (n=3 wells each group) and western blot analysis of the hCINAP and Flag-hCINAP expression. (d) Quantification of invaded cells in the transwell assay (n=3). (e) Tumorsphere formation assay using CRCSCs transfected as indicated. Scale bar, 100 μm. (f) Quantification of the tumorsphere formation assay (n=3). Asterisks indicate significant differences (*P<0.05, **P<0.01, ***P<0.001) determined by Student's t-test (a,c,d,f). NS, not significant.

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