Figure 3: Opposite effects of IRF1 and IRF4 on histone acetylation at Il9 locus. | Nature Communications

Figure 3: Opposite effects of IRF1 and IRF4 on histone acetylation at Il9 locus.

From: Reciprocal regulation of the Il9 locus by counteracting activities of transcription factors IRF1 and IRF4

Figure 3

(a–d) Purified WT and (a,b) Irf4−/− or (c,d) Irf1−/− CD4+ T cells were cultured under Th9 conditions with or without IFN-γ. (a,c) ChIP analysis of histone H4 acetylation (Ac) at CNS of the Il9 gene. (c) Bars show fold induction of H4-Ac relative to WT Th9 cells treated with IFN-γ. (b,d) ChIP analysis for RNA polymerase II (pol II) occupancy at Il9 and Rpl32 genes. (a–d) The same chromatin was used for control ChIP experiments with control IgG. Precipitated DNA is presented relative to input (% input). Values for nonspecific binding (as determined by using control IgG) were subtracted. (a–d) Data are shown as mean±s.d. of combined results from two independent experiments (triplicates to quintuplets in qRT-PCR). The experiments were repeated (b) three or (d) five times with consistent results. *P<0.05, **P<0.005, ***P<0.001 (two-tailed Student’s t-test).

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