Figure 3: rTWEAK induces skin inflammation.

(a,b) Naive WT mice were injected with 75 μg mouse rTWEAK or IgG, administered s.c. either four times during a 14 day period (a), or once with analysis on day 4, or twice weekly with analysis on day 10 (b,c). (a) Masson’s trichrome staining of representative skin sections on day 14. Scale bar, 200 μm. (b,c) Epidermal and dermal thickness on days 4, 10 and 14. Results are combined from two independent experiments. (d) Absolute number of skin-infiltrating cells measured by flow cytometry on day 4, and representative flow plot of skin-infiltrating eosinophils (SigF⁺CD45⁺CD11b⁺Ly6G⁻ cells). Combined from two experiments. Each data point one animal. Bars represent mean values. The ANOVA test with Bonferroni Correction was performed to compare the groups. Asterisks indicate P<0.05. n.s. indicates nonsignificant differences. ANOVA, analysis of variance.