Figure 2: CM-272 inhibits cell proliferation and induces apoptosis. | Nature Communications

Figure 2: CM-272 inhibits cell proliferation and induces apoptosis.

From: Discovery of first-in-class reversible dual small molecule inhibitors against G9a and DNMTs in hematological malignancies

Figure 2

(a) GI50 values of CM-272 for CEMO-1 ALL cell line, MV4-11 AML cell line and OCI-Ly10 DLBCL cell line. (b) H3K9me2 and 5mC levels after CM-272 treatment with different doses for 48 h in CEMO-1 cell line. H3 total was used as loading control. (c) H3K9me2 and 5mC levels after CM-272 treatment with different doses for 48 h in MV4-11 cell line. H3 total was used as loading control. (d) H3K9me2 levels after CM-272 treatment with different doses for 48 h in OCI-Ly10 cell line. H3 total was used as loading control. (e) Cell proliferation time course in CEMO-1, MV4-11 and OCI-Ly10 cell lines treated with three different concentrations of CM-272 (GI25, GI50 and GI75) for 12, 24, 48 and 72 h. (f) Cell cycle in CEMO-1, MV4-11 and OCI-Ly10 cell lines treated with three different concentrations of CM-272 (GI25, GI50 and GI75) for 24 h (a representative example of three different experiments is shown). (g) Apoptosis time course in CEMO-1, MV4-11 and OCI-Ly10 cell lines treated with three different concentrations of CM-272 (GI25, GI50 and GI75) for 12, 24, 48 and 72 h. Error bars indicate s.d. from three replicates.

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