Figure 5: SSNMR dipolar recoupling curves reveal exon1 fibril domain motion. | Nature Communications

Figure 5: SSNMR dipolar recoupling curves reveal exon1 fibril domain motion.

From: Fibril polymorphism affects immobilized non-amyloid flanking domains of huntingtin exon1 rather than its polyglutamine core

Figure 5

(a) Reference PDSD buildup profiles for one-bond Cα–Cβ cross-peaks of the crystalline dipeptide N-acetyl-Val-Leu, reflecting an example of a fully rigid molecule. The dashed line illustrates the lack of buildup for a fully mobile (for example, dissolved) molecule. Intermediate motion is expected to lead to build-up curves in-between these extremes. (b) PDSD buildup profiles for Cα–Cβ peaks of type-‘a’ Gln in the polyQ core, (c) α-helical A10 in httNT, random coil Ala (ARC) and (d) the random coil (RC) and PPII-structured Pro in the PRD of htt exon1 fibrils formed at 22 °C. Pale grey lines show the reference curves from a. Error bars indicate the s.d., as described in the Methods. The NMR measurements were performed at 275 K on a 600 MHz (1H) spectrometer.

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