Figure 6: Control of type-one errors for continuous traits in the stage 1 assessment of genotype and stage 2 assessment of genotype*sex interaction.

Resampling studies of wildtype data, to build data sets with ‘mock’ knockout animals under various phenotyping workflows, were used to assess the control of type-one errors for the statistical pipeline. (a) The stage 1 type-one error rate at a trait level at the 0.05 significance threshold as a function of workflow. (b) The stage 1 average type-one error rate for various significance thresholds and workflows. (c) The stage 2 type-one error rate at a trait level at the 0.05 significance threshold as a function of workflow. (d) The stage 2 average type-one error rate at a trait level for various significance thresholds and workflows. For a–d the one-batch workflow is represented by either B1 or a black line, a two batch workflow is represented by B2 or a red line, a three batch workflow is represented by B3 or a green line, a multiple batch workflow is represented by MB or an orange line, a dashed line represents the ideal rate, and finally a random workflow is represented by R or a blue line.