Figure 4: EC-specific Raf1L613V expression causes cardiac hypertrophy. | Nature Communications

Figure 4: EC-specific Raf1L613V expression causes cardiac hypertrophy.

From: Cellular interplay via cytokine hierarchy causes pathological cardiac hypertrophy in RAF1-mutant Noonan syndrome

Figure 4

(a) Representative gross morphology and H&E-stained sections of Tie2-L613V and control hearts at 10 weeks (scale bar, 1 mm), and HW/BW of Tie2-L613V and Nfatc1-L613V mice, measured at 4 months (mean±s.e.m.; n=27 (Tie2-control), 13 (Tie2-L613V), 15 (Nfatc1-control) or 8 (Nfatc1-L613V); ***P<0.0001, two-tailed Student’s t-test). (b) Representative WGA-stained cross-sections of 10-week-old Tie2-L613V and control hearts (original magnification, × 400; scale bars, 50 μm). Cross-sectional area (right), quantified using ImageJ (mean±s.e.m.; n=5 samples per group, with 200 CMs counted per sample; **P<0.005, *P<0.05, two-tailed Student’s t-test). (c) Representative echocardiograms of hearts from 4-month-old mice. Arrows indicate LV diastolic dimensions. (d) LVIDs and LVIDd and (e), LVPWd, measured by echocardiography at 4 months (mean±s.e.m.; n=24 (Tie2-control), 12 (Tie2-L613V), 15 (Nfatc1-control) or 9 (Nfatc1-L613V); **P<0.005, ***P<0.001, two-tailed Student’s t-test). (f) Cardiac contractility of 4-month-old mice, measured by invasive hemodynamics (mean±s.e.m.; n=27 (Tie2-control), 13 (Tie2-L613V), 15 (Nfatc1-control) or 8 (Nfatc1-L613V); statistical significance was assessed using two-tailed Student’s t-test).

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