Figure 3: PHF5A-Y36C protects against splicing modulator induced mis-splicing. | Nature Communications

Figure 3: PHF5A-Y36C protects against splicing modulator induced mis-splicing.

From: Splicing modulators act at the branch point adenosine binding pocket defined by the PHF5A–SF3b complex

Figure 3

(a) In vitro splicing assay in the presence of indicated splicing modulators in nuclear extracts containing WT or Y36C PHF5A. Error bar indicates s.d., n=4. (b) Taqman gene expression analysis of mature SLC25A19 mRNA levels and EIF4A1 pre-mRNA levels in either WT- or Y36C PHF5A-expressing cells treated with indicated splicing modulators. All data points were normalized to the corresponding DMSO-treated control samples and displayed in logarithmic scale on the y axis. Error bar indicates s.d., n=2.

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