Figure 4: USP13 deficient mice are more resistant to HSV-1 infection.
From: USP13 negatively regulates antiviral responses by deubiquitinating STING
(a) Survival (Kaplan-Meier curve) of Usp13+/+ (n=19) and Usp13m/m mice (n=16) intravenously injected with HSV-1 (2.5 × 106 PFU per mouse) monitored survival for 12 days. (b) ELISA analysis of IFN-α, IFN-β, IL-6 and CCL5 in the sera of Usp13+/+ and Usp13m/m mice (n=5) intravenously injected with HSV-1 (2.5 × 106 PFU per mouse) for 12 h. (c,d) qRT-PCR analysis of Ifnb, Tnf, or HSV-1 UL30 mRNA in the lungs (c, n=5) or histological analysis (H&E staining or Ly6C staining) of lung sections (d, n=3) from Usp13+/+ and Usp13m/m mice intravenously injected with HSV-1 (2.5 × 106) for 24 h. (e) qRT-PCR analysis of Ifnb, Ifna, and Tnf and plaque assays of the brains from Usp13+/+ and Usp13m/m mice (n=5) intraperitoneally injected with HSV-1 (5 × 106) for 4 days. *P<0.05; **P<0.01; ***P<0.001 (unpaired student’s t-test, b,c,e). Scale bars represent 100 μM. PFU, plaque-forming unit. Data are of combined three independent experiments (a) or representative of two (b–e) independent experiments (mean±s.d. in b,c,e).
