Figure 7: USP13 impairs the recruitment of TBK1 to STING.
From: USP13 negatively regulates antiviral responses by deubiquitinating STING
(a,b) Denature-IP (with anti-FLAG) and immunoblot analysis (with anti-FLAG, anti-HA or anti-GFP) of HEK293 cells transfected with plasmids encoding FLAG-STING, HA-tagged ubiquitin or mutations and either the empty vector or GFP-USP13 for 24 h. (c,d) Immunoprecipitation (with anti-STING) and immunoblot analysis (with anti-TBK1, anti-STING, anti-USP13 or anti-β-Actin) of THP-1 cells stably transfected with shUSP13 or shCon infected with HSV-1 for 0–12 h (c) or Usp13+/+ and Usp13m/m BMDCs infected with HSV-1 for 0–10 h (d). Data are representative of three independent experiments. See Supplementary Fig. 13 for uncropped immunoblots.
