Figure 2: Loss of inner centromere localized CPC weakens centromeric cohesion. | Nature Communications

Figure 2: Loss of inner centromere localized CPC weakens centromeric cohesion.

From: Inner centromere localization of the CPC maintains centromere cohesion and allows mitotic checkpoint silencing

Figure 2

(a,b) Cells were transfected with the indicated siRNAs, and synchronized according to the scheme (a), After the 45 min. accumulation in MG132 chromosome spreads were prepared (a) and the percentage of cells in which all chromosomes appeared as ‘X-shaped’, ‘railroads’, with ‘closed arms’, or in which all sister chromatids were fully separated, was quantified (b, n=2 exp., ≥185 cells per condition, error bars are s.e.m.). Representative images of the different categories are shown in a. Scale bars, 5 μm, resp. 2 μm in zoom. (c) Cells were transfected with siINCENP and treated according to the depicted scheme. After a 16 h monastrol, treatment cells were either released into MG132 (MG) or into nocodazole (N). Chromosome spreads were scored for the indicated chromosome appearance (n=2 exp., ≥210 cells percondition, error bars are s.e.m.).

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