Figure 3: SGO1 is predominantly localized at kinetochores in CB-INCENP expressing cells. | Nature Communications

Figure 3: SGO1 is predominantly localized at kinetochores in CB-INCENP expressing cells.

From: Inner centromere localization of the CPC maintains centromere cohesion and allows mitotic checkpoint silencing

Figure 3

(a) IF and quantification of fluorescence intensities (FI) of SGO1/CENP-C on centromeres and/or kinetochores in the depicted cell lines, transfected with the indicated siRNAs, and blocked in mitosis using STLC (1 exp. out of 2, 11 cells per condition, bars: mean±s.d., ns, not significant; *P<0.05; ****P<0.0001; unpaired t test). DNA is visualized using DAPI. Scale bar, 5 μm. (b) Representative sister kinetochores with SGO1, mCherry and CENP-C and 1D line graphs of SGO1 (green) and CENP-C (red) of cells transfected with the indicated siRNAs, blocked in mitosis using STLC and expressing INCENP variants that rescued SGO1 centromere/kinetochore localization (a). The percentage of kinetochore pairs showing that particular SGO1 staining pattern is shown on the right side of the images. Scale bar, 1 μm.

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