Figure 3: MiR-23b suppresses metastasis-relevant traits in vitro.
From: Genome-wide functional screening of miR-23b as a pleiotropic modulator suppressing cancer metastasis
(a) MiR-23b levels correlate inversely with mobility ability in human colon cancer cell lines, n=3. The migration (black bar) or invasion (grey bar) speed of SW620 was normalized to 1. (b) Migration (black bar) or invasion (grey bar) assays after transfection of HCT 116 cells with the indicated constructs, n=3. NC (scramble siRNA). (c) Anoikis assays with HCT 116 cells infected as indicated, n=3. NC (scramble siRNA). (d) Manipulation of the miR-23b levels can induce epithelial to mesenchymal transition. E-cadherin and vimentin are used as epithelial to mesenchymal transition markers. DAPI staining was used to detect nuclei. NC (scramble siRNA). Scale bar, 50 μm. (e) Western blot assays showing the expression levels of E-cadherin and vimentin in response to miR-23b. GAPDH is used as loading control. *P<0.05; **P<0.01 (t-test). Error bars represented s.d.'s of at least three independent experiments.
