Figure 5: MiR-23b directly regulates a cohort of prometastatic genes.
From: Genome-wide functional screening of miR-23b as a pleiotropic modulator suppressing cancer metastasis
(a) Luciferase activity in HCT 116 cells infected with miR-23b (white bar) or control vector (black bar) after transfection with the indicated 3′-UTR-driven reporter constructs, n=3. (b) Luciferase activity in HCT 116 cells infected with miR-23b or control vector after transfection with the indicated 3′-UTR-driven reporter constructs. Black bar: 3′-UTR+miR-23b; grey bar: mutation 3′-UTR+miR-23b; white bar: 3′-UTR+NC; n=3. (c) Immunoblots for endogenous Fzd7, MAP3K1, PAK2, RRAS2, TGF-βR2 and UPA in the indicated HCT 116 cells. GAPDH was use as the loading control. Repression: protein levels in miR-23b-expressing cells relative to vector controls were repressed. (d) Migration rescue (black bar) assays with the miR-23b-expressing HCT 116 cells transfected with individual expression constructs. Migration (grey bar) or anoikis resistance (white bar) assays with the HCT 116 cells transfected with the indicated siRNAs; n=3. (e) Images of epithelial to mesenchymal transition markers after transfection of HCT 116 cells infected as indicated. E-cadherin and vimentin are used as epithelial to mesenchymal transition markers. DAPI staining was used to detect nuclei. Scale bar, 50 μm. *P<0.05; **P<0.01; ***P<0.001 (t-test). Error bars represented s.d.'s of at least three independent experiments.
