Figure 6: MiR-23b inhibits tumour angiogenesis.
From: Genome-wide functional screening of miR-23b as a pleiotropic modulator suppressing cancer metastasis
(a) CD31-stained sections of primary tumours formed by HCT 116 cells infected as indicated (quantification below). Scale bar, 50 μm. (b) Tubule formation of endothelial cells infected as indicated. Shown is a representative image at 24 h after plating. Quantification of tubule branching and tubule length (below). Scale bar, 50 μm. (c) Real-time PCR for endogenous VEGF in the HCT 116 cells infected as indicated. GAPDH was used as the loading control. (d) Immunoblots for endogenous VEGF in the HCT 116 cells infected as indicated. GAPDH was used as the loading control. (e) Enzyme-linked immunosorbent assay for secreted VEGF in the HCT 116 cells infected as indicated. (f) Luciferase activity in HCT 116 cells infected with miR-23b or control vector after transfection of the VEGF 3′-UTR-driven reporter constructs; n=3. (g) Real-time PCR for endogenous VEGF in the HCT 116 cells infected as indicated. *P<0.05; **P<0.01; ***P<0.001 (t-test). Error bars represented s.d.'s of at least three independent experiments.
