Figure 5: phm-gal80 effectively suppresses tim-gal4 expression in the PG.
From: Central and peripheral clocks are coupled by a neuropeptide pathway in Drosophila

Pattern of GFP (left; a,d,g) and corresponding DAPI (right; c,f,i) and merged images (center; b,e,h) of pre-pupal brains (Br) expressing GFP under the control of tim-gal4 (a), tim-gal4+phm-gal80 (d) and phm-gal4 (g). Gain in a,b was set to visualize expression within the brain; arrowheads indicate LNs. Insets in a,b show expression in PG using the same gain used for g. In the ring gland, tim-gal4 drives expression in the PG and the corpora allata (labelled CA in a,d). Expression in the PG is suppressed when the tim-gal4 driver is combined with phm-gal80 (d). Scale bar (for a–i): 100μm.