Figure 8: Hierarchical relationship between the brain clock and the PG clock. | Nature Communications

Figure 8: Hierarchical relationship between the brain clock and the PG clock.

From: Central and peripheral clocks are coupled by a neuropeptide pathway in Drosophila

Figure 8

(ac) Pattern of emergence when all clocks (a) or only the brain (b) or the PG (c) clock have been slowed down. Schematic shown on the left represents the brain and PG clocks (large and small circle, respectively); orange colouring indicates which clock has been slowed down; middle: pattern of eclosion in DD; right: MESA analysis of record, with dominant periodicity indicated. (d) Average periodicities (±s.e.m.) for genotypes shown in ac and for controls; circles indicate periodicity for each separate experiment (N=5–8); average is indicated by horizontal line; different letters indicate statistically different groups (P<0.05; one-way ANOVA, Tukey’s post hoc multiple comparison analyses). (eg) Pattern of emergence when all clocks (e), or only the brain (f) or the PG (g) clock have been sped up (indicated in green). (h) Average periodicities (±s.e.m.) for genotypes shown in eg and for controls, represented as described in d. phm-gal80 effectively suppresses tim-gal4 expression in the PG (cf. Fig. 5d). (Strictly speaking, in (b,f) gene expression is driven in all clocks except the PG. However, since circadian rhythmicity of emergence depends only on the clocks in the brain and in the PG, such experiment is equivalent to driving gene expression only in the brain).

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