Figure 3: POP2 regulates the AIM2 inflammasome.
From: Pyrin-only protein 2 limits inflammation but improves protection against bacteria
(a) Mean level±s.d. of mature IL-1β measured from culture supernatants of pcDNA3 or POP2Tg J774A.1 transfectants treated with poly (dA:dT) (1 μg ml−1) for 18 h or (b) infected with F. novicida (MOI=100 for 24 h), L. monocytogenes (MOI=5 for 6 h) or S. Typhimurium (MOI=10 for 6 h) (n=3). (c) Immunohistochemical detection of Asc-speck formation (indicated by arrows) and per cent Asc specks in J774A.1 transfectants infected with F. novicida (MOI=100). (n>100 cells) (d) Mean level±s.d. of IL-1β and IL-18 measured from culture supernatants of BMDM from POP2Tg (n=4) and LMC (n=4) mice treated with poly (dA:dT) (1 μg ml−1) for 18 h. (e) Mean level±s.d. of mature IL-1β measured from culture supernatants of HEK293T cells transiently transfected with human ASC, AIM2 and/or POP2 constructs for 20 h (n=3). To determine significance, Student’s t-test was used. *P<0.05, ND, none detected.
