Figure 1: eVP30 binds eNP with high specificity.
From: Ebola virus VP30 and nucleoprotein interactions modulate viral RNA synthesis
(a) Constructs of eNP (top) and eVP30 (bottom) used in this study. (b) Summary of in vitro pull-down-based binding results for a series of truncation constructs for eNP. +, binding; −, no binding. (c) Size exclusion chromatography coupled to multi-angle light scattering results for eVP30110–272 (black; molecular weight (MW) 38.7±0.030 kDa), eNP600–739 (red; MW 16.8±0.030 kDa) and eVP30110–272-eNP600–739 complex (green; MW 39.6±0.030 kDa). The calculated MW for monomeric eVP30110–272, dimeric eVP30110–272 and eNP600–739 are 18.7, 37.3 and 17.03 kDa, respectively. The calculated MW for a 1:1 complex of eVP30110–272–eNP600–739 is 35.7 kDa. (d) Representative ITC binding isotherm for the 1:1 complex between eVP30110–272 and eNP600–739. KD=3.75±1.7 μM. (Also see Supplementary Fig. 1). Error represents s.d.
