Figure 5: Silencing of GhSWEET10 in cotton reduces water-soaking caused by Xcm infection.
From: TAL effector driven induction of a SWEET gene confers susceptibility to bacterial blight of cotton
(a) Xcm-mediated water-soaking lesion development on cotton plants upon VIGS. Cotyledons from 2-week-old Ac44E plants were syringe-infiltrated with Agrobacterium carrying a VIGS construct to silence GhSWEET10 (VIGS-GhSWEET10) or a GFP vector control (VIGS-Ctrl). Three weeks later, plants were vacuum infiltrated with XcmH1005 at OD600=0.01. Images were taken 2 weeks after inoculation. (b) Quantitative analysis of Xcm-mediated water-soaking lesions on cotton plants upon VIGS. Lesions were counted on the second true leaf of each VIGS plant (at least 10 plants were inoculated for each construct in each repeat). The data are shown as mean±s.e. (n=10). The asterisk indicates a significant difference with a Student’s t-test (P<0.05) when compared with the control. (c) RT–PCR analysis of GhSWEET10 expression in cotton plants upon VIGS. VIGS assays were done similarly as in a and 3 weeks later, the second true leaf was collected for RT–PCR analysis before Xcm inoculation. GhACTIN was used as an internal control. The above experiments were repeated three times with similar results.
