Figure 4: Spinal antinociception evoked by NAPQI, p-BQ and CA. | Nature Communications

Figure 4: Spinal antinociception evoked by NAPQI, p-BQ and CA.

From: TRPA1 mediates spinal antinociception induced by acetaminophen and the cannabinoid Δ9-tetrahydrocannabiorcol

Figure 4

Dose-dependent effects of intrathecal injections of NAPQI (a; vehicle in black, 70 pg in red, 700 pg in blue and 7 ng in cyan), p-BQ (b; vehicle in black, 250 pg in red, 25 ng in blue and 2.5 μg in cyan) and cinnamaldehyde (c; vehicle in black, 6.6 ng in red, 66 ng in blue, 660 ng in cyan and 66 μg in magenta) on the paw-withdrawal threshold in the hot-plate test in lightly restrained C57BL/6J mice. Data show mean±s.e.m. for 6 mice per group. *P<0.05; **P<0.01; ***P<0.001 compared with time-matched vehicle group. Effect of intrathecal injections of NAPQI (d; 7 ng), p-BQ (e; 2.5 μg) and cinnamaldehyde (f; 66 μg) in the hot-plate test in Trpa1+/+ mice (open circles) and Trpa1−/− mice (filled circles). Data show mean±s.e.m. for 6 mice per group. *P<0.05; **P<0.01; ***P<0.001 compared with pre-injection latency. All data analysed by ANOVA followed by Tukey's HSD test.

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