Figure 5: Increased signalling entropy in cancer cells and identification of drug resistant cancer stem cells.
From: Single-cell entropy for accurate estimation of differentiation potency from a cell’s transcriptome
(a) Boxplots of the signalling entropy (SR) for single melanoma cancer cells (c) compared to non-malignant (NotC) cells for three different melanoma patients (patient IDs given above each plot). Numbers of single cells are given below each boxplot. P value is from a Wilcoxon rank-sum test. (b) As a, but now pooled across all 12 patients. (c) Comparison of signalling entropy (SR) of 19 diagnostic acute myeloid leukaemia bulk samples to relapsed samples from the same patients. Wilcox rank sum test P value (one-tailed paired) is given. (d) Sorting of 96 single-AML cells from one patient according to signalling entropy and comparison of mRNA expression of AML CSC markers between low and high SR groups. P values from a one-tailed Wilcox test. (e) Comparison of signalling entropy (SR) of circulating tumour cells from metastatic prostate cancer patients who did not receive AR inhibitor treatment (UNTR) to those which developed resistance (RESIST). P value from a one-tailed Wilcox test. (f) Sorting of 73 single CTCs according to SCENT (signalling entropy, SR) into low and high SR groups. Correlation of gene expression of one putative CSC marker (ALDH7A1) with SR.
