Figure 5: Analysis of CD11c distribution relative to the medulla in Xcl1 mutants.

(a–d) Representative images of 6-week-old male Balb/c wild-type control (a,b; from N=5 thymi, with M=7, giving total degrees of freedom, NM=35) and Xcl1^−/− mutant (c,d; from N=4 thymi, with M=7, giving total degrees of freedom, NM=28) thymi reconstructed from multiple high-resolution images. The dendritic cell marker (CD11c) is green and the medullary marker (K14) is red. Clustering of the CD11c marker within the medulla is indicated by asterisks (*). (e–i) Graphical outputs from MiCASA: the log-spectra for the CD11c (c) and K14 (d) markers, the atanh-coherence (e) and the Variance plots for the K14 log-spectrum (f) and the atanh-coherence (g). Arrow in c indicates a single significant difference between the control and mutant log-spectra for CD11c. All MiCASA results are plotted as 99% confidence bands. (j–l) Examples of structures on intermediate (50–400 μm) scales, identified as boxed regions in b–d. Scale bars (a–d), 1,230 μm; (j,k), 220 μm.