Figure 1: Glucose represses GM-DCs expression of costimulatory molecules and IL-12.
From: Glucose represses dendritic cell-induced T cell responses
GM-DCs were left unstimulated (Unstim) or stimulated with LPS for 8 h, washed and then cultured in media containing either 10 mM glucose (Glc) or 10 mM galactose (Gal) (a–f) or in different concentrations of glucose (g). GM-DCs were then maintained in these culture conditions for up to 3 days as indicated. GM-DCs were analysed for (a) rates of glycolysis (ECAR) by seahorse analysis on day 1; by flow cytometry for (b,c) the expression of CD80 and CD86 costimulatory molecule expression, (d) cell viability by FSC/SSC analysis, (e) the expression of CD40 or (f,g) by qPCR on day 1 for IL12a and IL10 mRNA expression, as indicated. Data are mean±s.e.m. or representative of three (a,f), five (g) or six (b–d) separate experiments. qPCR performed in triplicate; seahorse analysis performed in quadruplicate. Data were analysed using a one-way analysis of variance with Tukey’s post test (*P<0.05, **P<0.01, ***P<0.001). MFI, mean fluorescent intensity.
