Figure 4: mTORC1 and HIF1α signalling is required for metabolic reprogramming in activated GM-DCs.
From: Glucose represses dendritic cell-induced T cell responses
(a,b) Hif1aflox/flox (WT) or Hif1aflox/flox VavCre (Hif1a−/−) GM-DCs were left unstimulated or stimulated with LPS (100 ng ml−1) for 20 h and analysed for rates of glycolysis (a) or by qPCR for the mRNA expression of the glucose transporter Slc2a1 and glycolytic enzymes phosphofructose kinase (Pkf) and Pyruvate kinase 2 (Pkm2) (b). (c,d) GM-DCs were left unstimulated or stimulated with LPS (100 ng ml−1)+/−rapamycin (Rapa, 20 nM) for 20 h and analysed for rates of glycolysis (c) or by qPCR for the mRNA expression Slc2a1 and Pkf and Pkm2 (d). Data are mean±s.e.m. at least three separate experiments performed in quadruplicate (a,c) or triplicate (b,d). Data were analysed using a one-way analysis of variance with Tukey’s post test (*P<0.05, **P<0.01, ***P<0.001). ECAR, extracellular acidification rate.
