Figure 5: RNA-seq data show downregulation decreases upon increasing genomic distance. | Nature Communications

Figure 5: RNA-seq data show downregulation decreases upon increasing genomic distance.

From: A damaged genome’s transcriptional landscape through multilayered expression profiling around in situ-mapped DNA double-strand breaks

Figure 5

(a) Plot showing the mean log fold-change of expression in induced vs uninduced samples upon increasing genomic distance. Mean logarithm of the fold change was measured for each set of genes located at a certain distance from the break and normalized by the total number of sites checked. Distance from the DSB is reported in Kb on the x axis. The diameter of each circle represents the number of genes considered (as reported in figure legend). Blue line represents linear fit to the data and grey-shaded area the s.e. (b) Coverage profile plot representing the log fold change of RNA-seq reads in induced vs uninduced samples for genes located at 0–0.1 Kb from an AsiSI site (yellow) and 1 Mb from an AsiSI site (violet). TES, transcription end site; TSS, transcription start site. Bold lines represent mean values, whereas semi-transparent shades around the mean curves represent the s.e.m. across the regions.

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