Figure 1: The microprotein Minion is specifically expressed during skeletal muscle development and regeneration.
From: The microprotein Minion controls cell fusion and muscle formation
(a) Left: Overlap of RNA-seq from regenerating adult mouse tibialis anterior (TA) muscle and differentiating C2C12 myoblasts (MB). CTX, cardiotoxin; FC, fold change compared to uninjured muscle (bottom left) or undifferentiated myoblasts (bottom right). Right: fold change of reads per kilobase per million mapped reads (RPKM) for selected genes upregulated after CTX injury. Values are normalized to uninjured muscle, representing mean±s.d. of fold change, three 8–10 week old mice per time point. (b) Western blot of control uninjured (Ctl) and CTX-injured regenerating adult TA muscle (n=2, two 8–10 week old mice per time point, two technical replicates each). (c) Western blot of embryonic muscle samples (n=3, two embryos each). Day 4 post-CTX TA or normal saline (NS) injection were positive and negative controls. E, embryonic day; P, post-natal. (d) Western blot of C2C12 myoblasts cultured under growth conditions (GM) or under differentiation conditions (DM) for the indicated number of days (n=4). MHC, myosin heavy chain. (b–d) Glyceraldehyde-3-phosphate dehydrogenase (Gapdh) and Tubulin served as loading controls. (e) Protein sequence alignment of mouse Minion with putative orthologues from other mammalian species (GenBank accession numbers and UniProt IDs from top to bottom are: NP_001170939.1, XP_017452417.1, NP_001302423.1, EHH18375.1, M3X8W7_FELCA and F1RQU5_PIG.)
