Figure 3: Muscle-specific expression and regulation of myomerger.
From: Myomerger induces fusion of non-fusogenic cells and is required for skeletal muscle development
(a) qRT-PCR for both Gm7325 long (L) and short (S) transcripts from various postnatal (P) day 5 tissues. (b–e) Immunoblotting for myomerger comparing P5 muscle to P28 muscle (b), WT to mdx4cv diaphragms (8 weeks of age) (c), sham plantaris to mechanically overloaded (MOV) plantaris (3 months of age) (d), and during C2C12 myoblast differentiation (e). GAPDH was used as a loading control. (f) Fractionation of C2C12 lysates on day 2 of differentiation followed by immunoblotting. (g) Representative immunostaining of fibroblasts infected with either empty, myomerger-short (S), or myomerger-long (L). Scale bar, 10 μm.
