Figure 7: Antigen-specific CD8⁺ and CD4⁺ T cell responses in naïve and DENV-immune macaques.

(a–l) All percentages shown are subtracted from the unstimulated background. (a–f) Analysis of the CD8⁺ T cell from the PBMCs of ZIKV-infected naïve and DENV-immune macaques (n=4 per cohort). PBMCs were harvested on day 30 and 60 p.i., and intracellular cytokine staining of IFN-γ (a), TNF-α (b) and CD107a (c) were analysed in CD8⁺ T cells after ex vivo re-stimulation with the listed stimuli. Intracellular cytokine staining of IFN-γ (d), TNF-α (e) and CD107a (f) were analysed for CD8⁺ T cells after ex vivo re-stimulation with the listed stimuli (for naïve group 30 d.p.i. n=3). (g–l) Analysis of the CD4⁺ T cell from the PBMCs of ZIKV-infected naïve and DENV-immune macaques (n=4 per group). PBMCs were harvested on day 30 and 60 p.i., and intracellular cytokine staining of IFN-γ (g), TNF-α (h) and CD107a (i) were analysed in CD4⁺ T cells after ex vivo re-stimulation with the listed stimuli. Intracellular cytokine staining of IFN-γ (j), TNF-α (k) and CD107a (l) were analysed in CD4⁺ T cells after ex vivo re-stimulation with the listed stimuli (for naïve group 30 d.p.i n=3).