Figure 2: Efficient gene transfection of adult DRG neurons.
From: Genetic dissection of axon regeneration via in vivo electroporation of adult mouse sensory neurons
(a) An image of an electroporated DRG that was dissected out with its nerve attached at 7 days after electroporation. (b) A representative image of an electroporated DRG showing EGFP-labelled neuronal cell bodies in the ganglion and their axons in the sciatic nerve and the dorsal root. Note that no EGFP-labelled axons were observed in the ventral root. (c) Enlarged images for the indicated areas (yellow arrows) of panel b. (d) An image of EGFP-labelled axonal terminals in the hind paw skin sections. (e) Representative images of an EGFP-transfected DRG immunostained with the neuronal marker βIII tubulin (red). (f) Representative images of the peripheral nerve segment near the EGFP-transfected ganglion that was cross-sectioned and immunostained with βIII tubulin (red). Scale bars, a,b, 500 μm; c, 125 μm; d, 100 μm; e,f, 200 μm.
