Figure 2: WASH affects the maintenance of NKp46+ ILC3s in a cell-intrinsic manner.
From: WASH maintains NKp46+ ILC3 cells by promoting AHR expression
(a,b) 2 × 106 Washflox/floxRORγt-GFP or Washflox/floxNcr1-CreRORγt-GFP BM cells were co-transplanted with 2 × 106 CD45.1 BM cells into lethally irradiated CD45.1 recipient mice, followed by donor chimerism examination of the indicated ILC3 subpopulations in small intestine (a) and large intestine (b) 8 wk later through flow cytometry (left panel). Donor chimerisms were calculated (right panel). For a and b, n=6. (c) Live NKp46+ ILC3 cells were sorted from mice reconstituted as in a through identifying surface markers, followed by Hoechst33342 and pyronin Y staining (left panel). Percentages of S/G2/M cells in the indicated cells were calculated (right panel). n=5. (d) Mice reconstituted with Washflox/floxRORγt-GFP or Washflox/floxRorc-CreRORγt-GFP BM cells were intraperitoneally injected with 7 mg kg−1 BrdU for 16 h, followed by flow cytometry analysis of BrdU signals in the indicated ILC3 cells. Percentages of BrdU positive cells were calculated. n=4. (e) Live ILC3 cells were sorted from mice reconstituted with Washflox/floxRORγt-GFP or Washflox/floxRorc-CreRORγt-GFP BM cells through identifying surface markers, followed by Hoechst33342 and pyronin Y staining. Percentages of S/G2/M cells in the indicated cells were calculated. n=4. Data are shown as means±SD. *P<0.05; **P<0.01; ***P<0.001. Data are representative of at least three independent experiments.
