Figure 6: Arid1a deficiency impedes AHR expression.

(a) Flow cytometry analysis of SI LPLs of Arid1a^flox/flox and Arid1a^flox/floxRorc-Cre mice (referred to as Arid1a^+/+ and Arid1a^−/−). Cells were gated from CD45⁺CD19⁻CD3⁻ cells (left panel). Numbers of ILC3s and the indicated subpopulations of ILC3s were calculated (right panel). (b) Arid1a^flox/flox and Arid1a^flox/floxRorc-Cre mice were intraperitoneally injected with 7 mg kg⁻¹ BrdU for 16 h, followed by flow cytometry analysis of BrdU signals in the indicated ILC3 cells. Percentages of BrdU positive cells were calculated. n=4. (c) Live ILC3 cells were sorted from Arid1a^flox/flox;RORγt-GFP and Arid1a^flox/floxRorc-Cre;RORγt-GFP mice through identifying surface markers, followed by Hoechst33342 and pyronin Y staining. Percentages of S/G2/M cells in the indicated cells were calculated. n=3. (d–f) Arid1a^flox/flox and Arid1a^flox/floxRorc-Cre BM cells transfected with full-length Arid1a or mutant Arid1a were co-transplanted with equal numbers of CD45.1 recipient BM cells into lethally irradiated mice, followed by examination of donor chimerism (d), AHR expression (e) and cell cycle (f) of NKp46⁺ ILC3s 8 week later. For d–f, n=7. Data are shown as means±s.d. *P<0.05; **P<0.01; ***P<0.001. Data are representative of at least three independent experiments.