Figure 4: Hepatocyte-specific senescence leads to fat accumulation in the liver.

(a) Six (n=3) and 12 month (n=5) old Alb-Xpg mice show increased numbers of hepatocytes positive for TAF. (b) Alb-Xpg mice display increased percentage of karyomegalic hepatocytes. (c) Representative images of p21 IHC staining in mouse liver. (arrows indicate positive cells and area in rectangle can be seen magnified at the right, scale bar=100 μm, brown=p21, blue=haematoxylin) (d) Immunohistochemistry staining shows significantly increased levels of p21-positive hepatocytes in 12-month-old Alb-Xpg mice. (e) Representative images of Oil Red O staining in the mouse liver (area in rectangle can be seen magnified at the right, scale bar=100 μm, red=Oil Red O, blue=haematoxylin). (f) Percentage of Oil Red O staining per area is significantly increased in Alb-Xpg mice at 12 months of age. All data are mean±s.d. with 3–5 animals per group. Significant differences (one-way analysis of variance) are indicated with *P≤0.05 and **P≤0.001.