Figure 3: Palmitoylation regulates VEGFR1 trafficking.
From: Dynamic alterations in decoy VEGF receptor-1 stability regulate angiogenesis
(a,b) (a) Immunofluorescence of VEGFR1 and PECAM or (b) VEGFR1 and Rab11a in HUVEC with indicated treatments; 2-BH, 2-bromohexadecadnoic acid, scale bars: 20 and 10 μm for a,b, respectively. (c,e) Mander’s Correlation Coefficient quantification of overlap of VEGFR1 and PECAM (c) or Rab11a (e) in HUVEC treated as indicated. (No. of cells: VEGFR1/PECAM: EtOH, n=14; 2-BH, n=12; VEGFR1/Rab11: EtOH, n=23; 2-BH, n=27); three replicates. (d) Immunoblot of cell surface or intracellular VEGFR1 from HUVEC with indicated treatments. PKM1/2, cytoplasmic marker; H-Ras, specificity control. Statistics: Shown are means+95% CI. Student’s t-test with post-hoc Tukey’s range test. **P≤0.01.
