Figure 5: Endothelial GPCR pattern after acute inflammatory activation by LPS in vivo. | Nature Communications

Figure 5: Endothelial GPCR pattern after acute inflammatory activation by LPS in vivo.

From: Single-cell profiling reveals heterogeneity and functional patterning of GPCR expression in the vascular system

Figure 5

(a–d) Analyses in brain EC (ECbr): (a) Heat map of GPCR expression in ECbr from healthy mice and LPS-treated mice (ECbrLPS) (52 and 22 cells from seven and four mice, respectively). Horizontal bars on the right side visualize expression frequency (in %) (for full data set, Supplementary Fig. 6); function-defining genes are shown in blue. (b) Average number of GPCRs expressed in individual ECbr from healthy or LPS-treated mice. (c) Heat map indicating dissimilarities between individual ECbr. K-means clustering identified two cell clusters that are colour-coded along the axes and correspond to ECbr from healthy and LPS-treated mice, respectively. (d) Fold difference in gene expression in ECbrLPS compared to all cells. (e–g) Analyses in lung EC (EClu) (48 and 25 cells from eight and four mice, respectively): (e) Heat map indicating dissimilarities between individual EClu. K-means clustering identified two cell clusters that are colour-coded along the axes and correspond to EClu from healthy and LPS-treated mice, respectively. (f) Fold difference in gene expression in ECluLPS compared to all cells. (g) Average number of GPCRs expressed in individual EClu from healthy or LPS-treated mice. (h–j) Comparison of LPS effects in EClu and ECbr: (h) T-SNE plot of k-means clustering data for different EC types with and without LPS treatment: cluster assignment is indicated by coloured numbers, cell type is indicated by symbol (each dot one cell; distance between dots indicates degree of similarity). (i,j) Comparative analysis of expression strength of selected GPCRs in different EC types. (k) Rearranged and extended heat map of ECbrLPS shown in a: Fpr1/Fpr2/Ccr1/C5ar1-expressing cells are indicated by red box. All expression data are calculated as 2(Limit of detection(LoD) Ct—sample Ct); LoD Ct was set to 24. Function-defining genes are shown in blue. Data in b,g,i,j are means±s.e.m.; comparisons were made using two-sample t-test. *P<0.05; **P<0.01; ***P<0.001.

Back to article page