Figure 3: Aggresomes containing misfolded polypeptides are dispersed into small cytoplasmic aggregates by downregulation of CED components.
From: Misfolded polypeptides are selectively recognized and transported toward aggresomes by a CED complex
(a) Immunostaining of polypeptidyl-puro using α-puromycin antibody. HeLa cells were transfected with the indicated siRNA. Two days later, the cells were treated with either DMSO (left) or MG132 (right) for 12 h, and with puromycin for 1 h before cell staining. Scale bar, 20 μm. (b) Effect of CED downregulation on the formation of aggresomes containing misfolded polypeptidyl-puro. The cells containing aggresomes and dispersed aggregates of misfolded polypeptidyl-puro in a were counted. The relative percentage of cells containing dispersed aggregates was calculated. (c) Effect of CED downregulation on the formation of aggresomes containing misfolded CFTR-ΔF508. As performed in a,b, except that the cells containing aggresomes, and dispersed aggregates of CFTR-ΔF508 were counted. Immunostained images are shown in Supplementary Fig. 6a. n=2. *P<0.05; **P<0.01.
