Figure 4: N-terminal region spanning amino-acid residues 12–53 of CTIF is critical for CED complex integrity and aggresomal targeting.
From: Misfolded polypeptides are selectively recognized and transported toward aggresomes by a CED complex

(a) Schematic diagram of FLAG-tagged CTIF variants. CBP80-interacting region and MIF4G domain are specified by light grey and dark grey boxes, respectively. (b) CTIF domain mapping by IPs using N-terminal serial deletions of CTIF in HEK293T cells. (c) Immunostaining of FLAG-tagged CTIF variants (green) in HeLa cells in the presence of either DMSO or MG132. n=3. Scale bar, 10 μm.