Figure 5: Accumulation of misfolded polypeptides preferentially inhibits CT.
From: Misfolded polypeptides are selectively recognized and transported toward aggresomes by a CED complex
(a) Polysome fractionation analysis of MEF-eIF2α (A/A) cells. Cells transiently expressing GST-CTIF-WT or GST-CTIF(54–598) were treated with MG132 for the indicated time before harvesting. Each fraction was subjected to western blotting using the indicated antibodies. (b) Relative distributions of GST-CTIF-WT or GST-CTIF(54–598) in polysome fractions. The intensities of each band in a were quantitated, and then the relative levels of GST-CTIF-WT or GST-CTIF(54–598) in each fraction were calculated as a percentage of the total. The dots and error bars represent the mean and s.d. of two biological replicates.
