Figure 1: USP13 regulates RAP80-BRCA1 foci formation.
From: USP13 regulates the RAP80-BRCA1 complex dependent DNA damage response
(a) A panel of deubiquitinases was knocked down in EFO-27 cells and cisplatin-induced BRCA1 foci were assayed. (b) USP13 was knocked out in EFO-27 cells by CRISPR and western blot was performed with the indicated antibodies. (c) Control or USP13 knockout EFO-27 cells were treated with cisplatin and foci formation of the indicated factors were detected by immunofluorescence with indicated antibodies. Representative images are shown in the upper panels. Scale bar, 10 μm. Quantification of the percentage of cells displaying foci formation is shown in the lower panels. (a,c) Error bars represent±s.e.m. from three independent experiments. **P<0.01. More than 200 cells were counted per experiment. Statistical analyses were performed with the ANOVA. ANOVA, analysis of variance.
