Figure 2: USP13 interacts with and deubiquitinates RAP80.
From: USP13 regulates the RAP80-BRCA1 complex dependent DNA damage response
(a) EFO-27 cell lysates were subjected to immunoprecipitation with control IgG or RAP80 antibodies. The immunoprecipitates were then blotted with the indicated antibodies. (b) Cell lysates from control (Ctrl) or USP13 knockout cells were subjected to immunoprecipitation with control IgG or USP13 antibodies. The western blots were then blotted with the indicated antibodies. (c) Control, USP13 knockout, and USP13 knockout cells stably expressing the indicated constructs were lysed under denaturing conditions and RAP80 was immunoprecipitated. Blots were probed with the indicated antibodies. (d) Control and USP13 knockout cells treated with or without Spautin-1 were lysed under denaturing conditions and RAP80 was immunoprecipitated. Blots were probed with the indicated antibodies. (e) Ubiquitinated HA-RAP80 was incubated with purified USP13 or USP13 CA in vitro and then blotted with the indicated antibodies. (f) Control or USP13 knockout cells were transfected with indicated His-Ub lysine-specific mutant constructs, cells were lysed under denaturing conditions and RAP80 was immunoprecipitated. Blots were probed with the indicated antibodies.
